Analysis of tumor samples from patients with metastatic colorectal cancers, treated with an Irinotecan/5-FU chemotherapy, using DNA arrays to determine genes important in tumor response to therapy.
Establishment of adenocarcinoma cell lines with in vitro acquired resistance to drugs (SN38, oxaliplatine, 5-FU).
A new and original approach, using intracellular antibodies, to screen for molecules able to modulated with a high specificity signaling pathways.

The team is focused on the study of the molecular mechanisms leading to chemotherapy resistance of cancer cells and on the development of new methods to isolate active compounds with a high specificity and low side effects.

In the proposed team, we have been joined by the team "Microtubule Dynamics in normal and tumor tissues” (INSERM E229), headed by C. Larroque. We decided to work together because of our common interest in drug mechanisms and targets and because of the complementary approaches followed. In addition, both groups have always tried to setup studies from patient to patient and we will continue in this direction through a even stronger collaboration with clinical teams and the arrival of Prof. M. Ychou in the team.